Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection

SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent...

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Bibliographic Details
Published in:Scientific reports 2021-05, Vol.11 (1), p.10716-10716, Article 10716
Main Authors: Piñero, Paula, Marco De La Calle, Francisco M, Horndler, Lydia, Alarcón, Balbino, Uribe Barrientos, Marisol, Sarmiento, Héctor, Tarín, Fabián
Format: Article
Language:English
Subjects:
631/1647
631/250
Colorimetry
COVID-19
Enzyme-linked immunosorbent assay
Flow cytometry
Humanities and Social Sciences
Immune response
Immune response (humoral)
Immunoglobulin A
Immunoglobulin G
multidisciplinary
Pandemics
Science
Science (multidisciplinary)
Serological tests
Serology
Severe acute respiratory syndrome coronavirus 2
Spike glycoprotein
Vaccination
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Summary:SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-90054-4