Use of Biologically Active 3D Matrix for Extensive Skin Defect Treatment in Veterinary Practice: Case Report

Large full-thickness skin defects represent a serious veterinary problem. We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth fa...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in veterinary science 2019-03, Vol.6, p.76-76
Main Authors: Zakirova, Elena Yu, Shalimov, Dmitry V, Garanina, Ekaterina E, Zhuravleva, Margarita N, Rutland, Catrin S, Rizvanov, Albert A
Format: Article
Language:English
Subjects:
3D matrix
FGF2
fibrin glue
mesenchymal stem cells
VEGF165
Veterinary Science
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Large full-thickness skin defects represent a serious veterinary problem. We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth factor two (FGF2; construct Ad5-VEGF165 + Ad5-FGF2) or multipotent mesenchymal stem cells, genetically modified with these constructs. studies confirmed the biosynthesis of VEGF165 and FGF2 mRNA in the transduced cells. Ad5-VEGF165 + Ad5-FGF2- transduced multipotent mesenchymal stem cells showed an enhanced capacity to form capillary-like tubes . Bioactive 3D-matrix application enhanced granulation tissue formation and epithelialization of non-healing, large bite wounds in a dog. Successful wound healing was observed with a positive clinical outcome for the canine patient. This research and application of regenerative gene therapy alongside a novel bioactive 3D-matrix shows promising clinical applications for the future in both dogs and other mammals including humans.
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2019.00076