Dietary fiber pectin alters the gut microbiota and diminishes the inflammatory immune responses in an experimental peach allergy mouse model

• Published in: Scientific reports 2024-12, Vol.14 (1), p.30503-13, Article 30503
• Main Authors: Steigerwald, Hanna, Albrecht, Melanie, Blissenbach, Birgit, Krause, Maren, Wangorsch, Andrea, Schott, Maike, Gonzalez-Menendez, Irene, Quintanilla-Martinez, Leticia, Toda, Masako, Vieths, Stefan, Krut, Oleg, Scheurer, Stephan, Blanco-Pérez, Frank
• Format: Article
• Language: English
• Subjects: 631/250; 631/250/249; 631/45/72/1201; 692/308/1426; 692/308/2778; 692/699/1503; 692/699/1702; 692/699/249; Animals; Dietary fiber; Dietary Fiber - pharmacology; Disease Models, Animal; Feces - microbiology; Female; Food Hypersensitivity - immunology; Food Hypersensitivity - prevention & control; Gastrointestinal Microbiome - drug effects; Gut microbiota; Humanities and Social Sciences; Immune modulation; Immunoglobulin E - blood; Immunoglobulin E - immunology; Inflammation - immunology; Mice; multidisciplinary; Peach allergy; Pectin; Pectins - pharmacology; Prunus persica - immunology; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-82210-3

Since therapeutic options are limited the utilization of prebiotics is suggested to prevent food allergies (FAs). Using an experimental peach allergy model we explored the effect of dietary fiber pectin, a high-methoxyl heteropolysaccharide, on the manifestation of FA. CBA/J mice were sensitized, subsequently orally boosted and provoked with peach peel extract. For dietary intervention, mice were fed a pectin containing diet before (primary-preventive) or after (secondary-preventive) sensitization. Non-treated allergic and sham-treated mice were fed a diet containing 20% cellulose. Fecal microbiota, humoral and intestinal immune cell responses were analyzed. Pectin remarkably affected the gut microbiota composition and diversity, promoting mainly the growth of Bacteroides . The frequency of mast cells, macrophages, and CD3 + T cells in the lamina propria of the small intestine was reduced, whereas the frequency of B cells and CD4 + T cell subpopulation was enhanced. Pectin intervention in the primary-preventive stetting significantly triggered serum IgA levels, whereas production of IgE and mMCPT-1 was reduced. Remarkably, in both settings peach allergen-specific IgG1/IgG2a ratio and specific IgE were significantly reduced to baseline. The data suggest, that dietary supplementation of pectin in both intervention approaches can diminish inflammatory responses and signs of allergic immune responses, accompanied by alteration of the gut microbiota composition.