Prevention of rocuronium induced mast cell activation with prophylactic oleuropein rich diet in anesthetized rabbits

• Published in: Acta cirúrgica brasileira 2018-11, Vol.33 (11), p.954-963
• Main Authors: Simsek, Tuncer, Erbas, Mesut, Buyuk, Basak, Pala, Cigdem, Sahin, Hasan, Altinisik, Betul
• Format: Article
• Language: English
• Subjects: Anesthesia; Mast Cells; Rabbits
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/s0102-865020180110000002

Abstract Purpose: The effect of a prophylactic oleuropein-rich diet before anesthesia accompanied by the widely-used steroid-based neuromuscular drug rocuronium on mast cell activation was investigated in the study. Methods: 14 rabbits used in the study. The rabbits in the oleuropein group were given oleuropein-rich extract added to the animals’ water at doses of 20 mg/kg oleuropein for 15 days orally. After 15 days, all rabbits in the two groups were given general anesthesia with rocuronium of 1 mg/kg. After 1 day, animals were sacrificed and the liver tissue sections stained with H&E, toluidine blue and tryptase for immunohistochemical study. Results: There was no statistically significant difference between ALT, AST and albumin averages of the oleuropein and control groups (p> 0.05). The tryptase average of the control group was higher than the tryptase average of the oleuropein group and this difference was statistically significant (p=0.003). The T. blue average in the oleuropein group was higher than the control group. However, there was no statistically significant difference between groups (p=0.482). Conclusions: Rocuronium adverse effects, like hypersensitivity and anaphylaxis, may limit routine use of this substance. The use of oleuropein reduced the number of inflammatory cells and prevented degranulation.