Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats

• Published in: Brain, behavior, & immunity. Health behavior, & immunity. Health, 2021-07, Vol.14, p.100256, Article 100256
• Main Authors: Wang, Yijie, Jaggers, Robert M., Mar, Pamela, Galley, Jeffrey D., Shaffer, Terri, Rajab, Adrian, Deshpande, Shivani, Mashburn-Warren, Lauren, Buzzo, John R., Goodman, Steven D., Bailey, Michael T., Besner, Gail E.
• Format: Article
• Language: English
• Subjects: Cognitive function; Full Length; Lactobacillus reuteri; Learning; Memory; Necrotizing enterocolitis (NEC); Probiotics
• ISSN: 2666-3546; 2666-3546
• DOI: 10.1016/j.bbih.2021.100256

Necrotizing enterocolitis (NEC) is a devastating disease affecting premature newborns with no known cure. Up to half of survivors subsequently exhibit cognitive impairment and neurodevelopmental defects. We created a novel probiotics delivery system in which the probiotic Lactobacillus reuteri (Lr) was induced to form a biofilm [Lr (biofilm)] by incubation with dextranomer microspheres loaded with maltose (Lr-DM-maltose). We have previously demonstrated that a single dose of the probiotic Lr administered in its biofilm state significantly reduces the incidence of NEC and decreases inflammatory cytokine production in an animal model of the disease. The aim of our current study was to determine whether a single dose of the probiotic Lr administered in its biofilm state protects the brain after experimental NEC. We found that rat pups exposed to NEC reached developmental milestones significantly slower than breast fed pups, with mild improvement with Lr (biofilm) treatment. Exposure to NEC had a negative effect on cognitive behavior, which was prevented by Lr (biofilm) treatment. Lr administration also reduced anxiety-like behavior in NEC-exposed rats. The behavioral effects of NEC were associated with increased numbers of activated microglia, decreased myelin basic protein (MBP), and decreased neurotrophic gene expression, which were prevented by administration of Lr (biofilm). Our data indicate early enteral treatment with Lr in its biofilm state prevented the deleterious effects of NEC on developmental impairments. •Early treatment with Lr in its biofilm state improves cognitive function in pups that survive experimental NEC.•Lr in its biofilm state reduces microglia activation and MBP loss, and maintains memory and learning-related gene expression.•Administration of Lr in its biofilm state protects the brain, as well as intestines, during experimental NEC.