The Survival of Pear Dormant Buds at Ultra-Low Temperatures

Currently, there is a varietal diversity decline in pear orchards of the Czech Republic. Thus, the safe storage of their gene pool collections is becoming increasingly important. Therefore, the ultra-low temperature survival after two-step cryopreservation treatment of dormant buds was tested for a...

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Bibliographic Details
Published in:Plants (Basel) 2021-11, Vol.10 (11), p.2502
Main Authors: Bilavcik, Alois, Faltus, Milos, Zamecnik, Jiri
Format: Article
Language:English
Subjects:
Buds
Communication
Cryopreservation
Dehydration
dormant buds
Fruits
Gene pool
Germplasm
Low temperature
low-temperature resistance
Moisture content
Nitrogen
Orchards
pear tree
Regeneration
Survival
Water content
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Summary:Currently, there is a varietal diversity decline in pear orchards of the Czech Republic. Thus, the safe storage of their gene pool collections is becoming increasingly important. Therefore, the ultra-low temperature survival after two-step cryopreservation treatment of dormant buds was tested for a safe and rapid way to conserve pear germplasm in a broader range of varieties. The following varieties crucial for cultivation in the Czech Republic were tested; ‘Amfora’, ‘Beurré Hardy’, ‘Bosc’, ‘Clapp’s Favourite’, ‘Conference’, ‘Dicolor’, ‘Erika’, ‘Lucas’, ‘Williams’ and ‘Williams Red’. In 2011 and 2012, dormant pear buds were dehydrated to 40.1% and 36.0% water content, respectively, before cryopreservation. The average regeneration of the dormant pear buds after cryopreservation by the two-step cryoprotocol in 2011 and 2012 was 54.3% and 16.1%, respectively. The mentioned cryopreservation procedure is suitable for the safe storage of dormant buds in most tested pear varieties.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10112502