Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

• Published in: Memórias do Instituto Oswaldo Cruz 2006-02, Vol.101 (1), p.15-20
• Main Authors: Prestes-Carneiro, Luiz Euribel, Freitas, Selma de Bastos Zambeli de, Zago, Sueli Cristina Schadeck, Miguel, Nadia Araújo, Primo, Oswaldo Bill, Iha, Alberto Hiroshi, Espíndola, Noeli Maria, Vaz, Adelaide José
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Animals; Antibodies, Helminth - blood; Biomarkers - blood; Brazil; Brazil - epidemiology; Child; Cysticercosis - diagnosis; Cysticercosis - epidemiology; enzyme linked immunoabsorbent assay; Enzyme-Linked Immunosorbent Assay; Feces - parasitology; Female; Humans; IgE; immunoblot; Immunoblotting; Immunoglobulin E - blood; Luminescent Measurements; Male; Middle Aged; PARASITOLOGY; Taenia solium cysticercus antibodies; Taenia solium cysticercus antibodies - enzyme linked immunoabsorbent assay - immunoblot - IgE - Brazil; Taeniasis - diagnosis; Taeniasis - epidemiology; TROPICAL MEDICINE
• ISSN: 1678-8060; 0074-0276; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762006000100004

In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52°36'12", latitude 22°17'12") a series of laboratory markers were determined. After signing an informed consent, participants answered a standardized questionnaire. To determine anti-Taenia solium cysticercus antibodies, the samples were tested by enzyme linked immunoabsorbent assay using 18-and 14-kDa antigen proteins from vesicular fluid of Taenia crassiceps (VF-Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Total IgE levels were determined by chemmiluminescence's assay and hemogram by flow cytometer flux counter. A total of 84 individuals, 5.9% presented anti-T. solium cysticercus antibodies in ELISA and 3.6% were strongly reactive in the 18/14 kDa immunoblotting confirmatory test. All of the individuals with positive antibodies showed elevated Total IgE levels. We conclude that the frequency of anti-T. solium cysticercus antibodies in this population is higher than other regions considered endemic in São Paulo. Thus, it is important to carry out surveys in Peasants' settlement areas with the objective of establishing public health measures for prevention and control of infectious diseases such as taeniosis-cysticercosis.