Dynamic Assembly/Disassembly of Staphylococcus aureus FtsZ Visualized by High-Speed Atomic Force Microscopy

FtsZ is a key protein in bacterial cell division and is assembled into filamentous architectures. FtsZ filaments are thought to regulate bacterial cell division and have been investigated using many types of imaging techniques such as atomic force microscopy (AFM), but the time scale of the method w...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-02, Vol.22 (4), p.1697
Main Authors: Fujita, Junso, Sugiyama, Shogo, Terakado, Haruna, Miyazaki, Maho, Ozawa, Mayuki, Ueda, Nanami, Kuroda, Natsuko, Tanaka, Shun-Ichi, Yoshizawa, Takuya, Uchihashi, Takayuki, Matsumura, Hiroyoshi
Format: Article
Language:English
Subjects:
Atomic force microscopy
Bacteria
bacterial cell division
Bacterial Proteins - chemistry
Cell division
Comparative analysis
Cytoskeletal Proteins - chemistry
Cytoskeleton - chemistry
E coli
Filaments
FtsZ
High speed
high-speed atomic force microscopy
Imaging techniques
Microscopy
Microscopy, Atomic Force - methods
Protein Conformation
Protein Multimerization
Staphylococcus aureus
Staphylococcus aureus - chemistry
Staphylococcus aureus - metabolism
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Summary:FtsZ is a key protein in bacterial cell division and is assembled into filamentous architectures. FtsZ filaments are thought to regulate bacterial cell division and have been investigated using many types of imaging techniques such as atomic force microscopy (AFM), but the time scale of the method was too long to trace the filament formation process. Development of high-speed AFM enables us to achieve sub-second time resolution and visualize the formation and dissociation process of FtsZ filaments. The analysis of the growth and dissociation rates of the C-terminal truncated FtsZ (FtsZt) filaments indicate the net growth and dissociation of FtsZt filaments in the growth and dissociation conditions, respectively. We also analyzed the curvatures of the full-length FtsZ (FtsZf) and FtsZt filaments, and the comparative analysis indicated the straight-shape preference of the FtsZt filaments than those of FtsZf. These findings provide insights into the fundamental dynamic behavior of FtsZ protofilaments and bacterial cell division.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22041697