Comparative transcriptome analysis reveals the impact of daily temperature difference on male sterility in photo-thermo-sensitive male sterile wheat

• Published in: BMC genomics 2024-07, Vol.25 (1), p.740-16, Article 740
• Main Authors: Niu, Fuqiang, Liu, Zihan, Liu, Yongjie, Bai, Jianfang, Zhang, Tianbao, Yuan, Shaohua, Bai, Xiucheng, Zhao, Changping, Zhang, Fengting, Sun, Hui, Zhang, Liping, Song, Xiyue
• Format: Article
• Language: English
• Subjects: Agricultural production; Analysis; Biosynthesis; Cost benefit analysis; Cutin; Daily temperature difference; Diseases and pests; Experiments; Fertility; Flavonoids; Flowers & plants; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene sequencing; Genes; Genetic aspects; Germplasm; Growth; High temperature; Hybrid systems; Impact analysis; Infertility, Male; Light; Low temperature; Male sterility; Males; MAP kinase; Methods; Morphology; Network analysis; Photoperiod; Photoperiods; Plant Infertility - genetics; Prevention; Propagation; R&D; Research & development; Risk factors; RNA sequencing; Safe seed production; Seeds; Signal transduction; Sucrose; Temperature; Temperature gradients; Transcriptome; Transcriptomes; Triticum - genetics; Triticum - growth & development; Triticum - metabolism; Wheat
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10627-1

Photo-thermo-sensitive male sterility (PTMS), which refers to the male sterility triggered by variations in photoperiod and temperature, is a crucial element in the wheat two-line hybrid system. The development of safe production and efficient propagation for male sterile lines holds utmost importance in two-line hybrid wheat. Under the stable photoperiod condition, PTMS is mainly induced by high or low temperatures in wheat, but the effect of daily temperature difference (DTD) on the fertility conversion of PTMS lines has not been reported. Here, three BS type PTMS lines including BS108, BS138, and BS366, as well as a control wheat variety J411 were used to analyze the correlation between fertility and DTD using differentially sowing tests, photo-thermo-control experiments, and transcriptome sequencing. The differentially sowing tests suggested that the optimal sowing time for safe seed production of the three PTMS lines was from October 5th to 25th in Dengzhou, China. Under the condition of 12 h 12 °C, the PTMS lines were greatly affected by DTD and exhibited complete male sterility at a temperature difference of 15 °C. Furthermore, under different temperature difference conditions, a total of 20,677 differentially expressed genes (DEGs) were obtained using RNA sequencing. Moreover, through weighted gene co-expression network analysis (WGCNA) and KEGG enrichment analysis, the identified DEGs had a close association with "starch and sucrose metabolism", "phenylpropanoid biosynthesis", "MAPK signaling pathway-plant", "flavonoid biosynthesis", and "cutin, and suberine and wax biosynthesis". qRT-PCR analysis showed the expression levels of core genes related to KEGG pathways significantly decreased at a temperature difference of 15 ° C. Finally, we constructed a transcriptome mediated network of temperature difference affecting male sterility. The findings provide important theoretical insights into the correlation between temperature difference and male sterility, providing guidance for the identification and selection of more secure and effective PTMS lines.