Transcriptome analysis of five different tissues of bitter gourd (Momordica charantia L.) fruit identifies full-length genes involved in seed oil biosynthesis

The bitter gourd seed oil, rich in conjugated fatty acids, has therapeutic value to treat cancer, obesity, and aging. It also has an industrial application as a drying agent. Despite its significance, genomics studies are limited, and the genes for seed oil biosynthesis are not fully understood. In...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2022-09, Vol.12 (1), p.15374-15374, Article 15374
Main Authors: Ravichandiran, Kumar, Parani, Madasamy
Format: Article
Language:English
Subjects:
631/208/212
631/208/2491
631/208/514
Aging
Biosynthesis
Drying
Fatty acids
Fruits
Genomics
Humanities and Social Sciences
Industrial applications
Medicinal plants
multidisciplinary
Oils & fats
Oilseeds
Science
Science (multidisciplinary)
Seeds
Transcriptomes
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The bitter gourd seed oil, rich in conjugated fatty acids, has therapeutic value to treat cancer, obesity, and aging. It also has an industrial application as a drying agent. Despite its significance, genomics studies are limited, and the genes for seed oil biosynthesis are not fully understood. In this study, we assembled the fruit transcriptome of bitter gourd using 254.5 million reads (Phred score > 30) from the green rind, white rind, pulp, immature seeds, and mature seeds. It consisted of 125,566 transcripts with N50 value 2,751 bp, mean length 960 bp, and 84% completeness. Transcript assembly was validated by RT-PCR and qRT-PCR analysis of a few selected transcripts. The transcripts were annotated against the NCBI non-redundant database using the BLASTX tool (E-value 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-19686-4