Mechanism of Ca2+ transport by ferroportin

Ferroportin (Fpn) is a transporter that releases ferrous ion (Fe 2+ ) from cells and is important for homeostasis of iron in circulation. Export of one Fe 2+ by Fpn is coupled to import of two H + to maintain charge balance. Here, we show that human Fpn (HsFpn) binds to and mediates Ca 2+ transport....

Full description

Saved in:
Bibliographic Details
Published in:eLife 2023-01, Vol.12
Main Authors: Shen, Jiemin, Wilbon, Azaan Saalim, Zhou, Ming, Pan, Yaping
Format: Article
Language:English
Subjects:
Binding sites
Biochemistry and Chemical Biology
calcium
Calcium homeostasis
Calcium transport
Calcium-binding protein
cryo-EM
Homeostasis
Iron
membrane transport
Mutation
SLC40
Structural Biology and Molecular Biophysics
structure
Variance analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ferroportin (Fpn) is a transporter that releases ferrous ion (Fe 2+ ) from cells and is important for homeostasis of iron in circulation. Export of one Fe 2+ by Fpn is coupled to import of two H + to maintain charge balance. Here, we show that human Fpn (HsFpn) binds to and mediates Ca 2+ transport. We determine the structure of Ca 2+ -bound HsFpn and identify a single Ca 2+ binding site distinct from the Fe 2+ binding sites. Further studies validate the Ca 2+ binding site and show that Ca 2+ transport is not coupled to transport of another ion. In addition, Ca 2+ transport is significantly inhibited in the presence of Fe 2+ but not vice versa. Function of Fpn as a Ca 2+ uniporter may allow regulation of iron homeostasis by Ca 2+ .
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.82947