Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicin...

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Bibliographic Details
Published in:International journal of molecular sciences 2020-10, Vol.21 (19), p.7005
Main Authors: Zhao, Conghui, Yu, Zhenming, Silva, Jaime A Teixeira da, He, Chunmei, Wang, Haobin, Si, Can, Zhang, Mingze, Zeng, Danqi, Duan, Jun
Format: Article
Language:English
Subjects:
Biosynthesis
Budding
Chloroplast Proteins - genetics
Chloroplast Proteins - metabolism
Chloroplasts
Chloroplasts - enzymology
Chloroplasts - genetics
Dendrobium - enzymology
Dendrobium - genetics
Dendrobium officinale
E coli
Enzymes
floral volatiles
Flowers
Flowers & plants
Flowers - enzymology
Flowers - genetics
Genes
Genetic modification
Genomes
geraniol
Localization
MEP pathway
Nicotiana - enzymology
Nicotiana - genetics
Odorants
Orchids
Phosphatase
Phosphoric Monoester Hydrolases - genetics
Phosphoric Monoester Hydrolases - metabolism
Phylogenetics
Physiological aspects
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Pollinators
Proteins
terpene synthase
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Summary:Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid has not yet been identified. In this study, three potential geraniol synthases were mined from the genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. was highly expressed in flowers, especially in petals. transcript levels were high in the budding stage of flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of in leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in or in other ornamental orchids.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms21197005