In vitro investigation of gastrointestinal stability and toxicity of 3-hyrdoxypropionaldehyde (reuterin) produced by Lactobacillus reuteri

• Published in: Toxicology reports 2021-01, Vol.8, p.740-746
• Main Authors: Soltani, Samira, Couture, Frédéric, Boutin, Yvan, Ben Said, Laila, Cashman-Kadri, Samuel, Subirade, Muriel, Biron, Eric, Fliss, Ismail
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; antimicrobial properties; biopreservatives; cell lines; colorimetry; Cytotoxicity; digestive tract; Eukaryotic cells; gastrointestinal system; gastrointestinal transit; hemolysis; In vitro digestion; lactate dehydrogenase; Lactobacillus reuteri; metabolites; Regular; Reuterin; therapeutics; toxicology; viability
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2021.03.025

[Display omitted] •Reuterin (3-hyrdoxypropionaldehyde (3-HPA)) is a highly potent metabolite of Lactobacillus reuteri.•Reuterin is highly stable in gastrointestinal condition.•Human colorectal adenocarcinoma cells’ viability and membrane integrity remained unaltered by reuterin.•No significant hemolytic activity was detected.•Reuterin is a promising therapeutic and/or food preservative. Reuterin (3-hyrdoxypropionaldehyde (3-HPA)) is a highly potent metabolite of L. reuteri, which has applications in food, health, and veterinary sectors. Similar to other natural antimicrobial compounds, the approval of reuterin as a bio-preservative or therapeutic agent by regulatory agencies relies on sufficient data on its cytotoxicity and behavior in the gastrointestinal environment. Although the antimicrobial activity of reuterin has been broadly studied, its safety and toxicity are yet to be explored in detail. In this study, the stability and activity of reuterin were investigated in the gastrointestinal tract using in vitro models simulating gastrointestinal conditions. In addition, hemolytic activity and in vitro cytotoxicity of reuterin were evaluated by neutral red assay and lactate dehydrogenase (LDH) colorimetric assay using the same cell line. Activity of reuterin was observed to be stable during gastrointestinal transit. Viability and membrane integrity of cells remained unaltered by reuterin up to 1080 mM concentration. Furthermore, no hemolysis was observed in blood cells exposed to 270 mM reuterin. This study provides unique and highly relevant in vitro data regarding gastrointestinal behavior and toxicity of reuterin. In conclusion, the current study indicates that within a certain concentration range, reuterin can be safely used in bio-preservation and therapeutics applications. However, further in vivo studies are required to confirm these findings.