The Role of MicroRNA Signature as Diagnostic Biomarkers in Different Clinical Stages of Colorectal Cancer

Colorectal cancer (CRC) is one of the most common cancers and a major cause of cancer-related death worldwide. The early diagnosis of colorectal tumors is one of the most important challenges in cancer management. MicroRNAs (miRNAs) have provided new insight into CRC development and have been sugges...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2018-07, Vol.20 (2), p.220-230
Main Authors: Eslamizadeh, Sara, Heidari, Mansour, Agah, Shahram, Faghihloo, Ebrahim, Ghazi, Hossein, Mirzaei, Alireza, Akbari, Abolfazl
Format: Article
Language:English
Subjects:
Biomarker
Biomarkers
Blood
Cancer
Carcinogenesis
Carcinogens
Colorectal Cancer
Colorectal carcinoma
Control methods
Diagnosis
Diagnostic systems
Gene expression
Genomics
Metastasis
MicroRNA
MicroRNAs
miRNA
Molecular chains
Molecular modelling
Mortality
Original
Plasma
Polymerase chain reaction
Ribonucleic acid
RNA
RNA-directed DNA polymerase
Statistical analysis
Statistical methods
Tissues
Tumors
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Summary:Colorectal cancer (CRC) is one of the most common cancers and a major cause of cancer-related death worldwide. The early diagnosis of colorectal tumors is one of the most important challenges in cancer management. MicroRNAs (miRNAs) have provided new insight into CRC development and have been suggested as reliable and stable biomarkers for diagnosis and prognosis. This study's objective was to analyze the differential expression of miRNAs at differentstages of CRC searching for possible correlation with clinicopathological features to examine their potential value as diagnostic biomarkers. In this case-control study, plasma and matched tissue samples were collected from 74 CRC patients at stage II-IV as well as blood samples from 32 healthy controls. After exhaustive study of the current literature, eight miRNAs including miR-200c, 20a, 21, 31,135b, 133b,145 and let-7g were selected. The expression level of the miRNAs was assayed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Statistical analysis, including t test , Mann-Whitney U, Kruskall-Wallis tests and receiver operating characteristic (ROC) curve was applied, where needed. Significantly elevated levels of miR-21, miR-31, miR-20a, miR-135b, and decreased levels of miR- 200c, miR-145 and let-7 g were detected in both plasma and matched tissue samples compared to the healthy group (P0.05). ROC for tissue miRNAs showed an area under the ROC curve (AUC) of 0.98 and P
ISSN:2228-5806
2228-5814
DOI:10.22074/cellj.2018.5366