Proteomics Research Reveals the Molecular Mechanism by Which Grape Seed Oil Inhibits Tuber Sprouting in Potato

Potato tubers are rich in starch, vitamins, protein, minerals, and other nutrients. However, tuber sprouting produces solanine and reduces the commodity value of potatoes during storage. At present, it is known that some plant essential oils can inhibit tuber sprouting. It has been reported that gra...

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Published in:Horticulturae 2023-08, Vol.9 (8), p.890
Main Authors: Lyu, Chengcheng, Zhang, Xing, Li, Xiang, Lu, Yifei, Yuan, Jichao, Lu, Liming, Wang, Qiang, Wang, Xiyao, Li, Liqin
Format: Article
Language:English
Subjects:
Energy metabolism
Environmental aspects
Enzymes
Essences and essential oils
Essential oils
Grape oil
grape seed oil
Grapes
Grapeseed oil
Growth
Inflammation
Lipids
Metabolism
Metabolites
Molecular modelling
Nitrogen
Nutrients
Nutritional aspects
Oils & fats
Oilseeds
Oxidation
Peptides
Peroxidase
Physiological aspects
potato
Potatoes
Proteins
Proteomics
Seeds
sprouting
Statistical analysis
Transaminase
tuber
Vegetables
Vitamins
γ-Aminobutyric acid
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Summary:Potato tubers are rich in starch, vitamins, protein, minerals, and other nutrients. However, tuber sprouting produces solanine and reduces the commodity value of potatoes during storage. At present, it is known that some plant essential oils can inhibit tuber sprouting. It has been reported that grape seed oil (GSO) has antioxidant, anti-inflammatory, and anticancer characteristics, reducing blood lipids and delaying aging. In this study, we found for the first time that GSO delayed tuber sprouting, and the soluble sugar content and peroxidase activity changed after 60 days of GSO treatment. Furthermore, a comparative proteomic analysis of tuber bud eyes showed that after 30 days of GSO treatment, there were 206 and 129 differentially abundant proteins (DAPs) with increased and decreased abundance levels, respectively. After analysis, we found that 15 ROS-related proteins and 14 proteins involved in energy metabolism were DAPs. Among them, gamma aminobutyrate transaminase 1 had decreased abundance after GSO treatment. Meanwhile, the transcription level of genes related to GABA synthesis increased significantly according to qRT-PCR analysis. Our results provide new approaches to the proteomic mechanism of potato sprouting after GSO treatment and provide a theoretical basis for the application of GSO in inhibiting potato seed sprouts.
ISSN:2311-7524
2311-7524
DOI:10.3390/horticulturae9080890