Comparison of immune response to human rhinovirus C and respiratory syncytial virus in highly differentiated human airway epithelial cells

Human rhinovirus C (HRV-C) accounts for a large proportion of HRV-related illnesses, but the immune response to HRV-C infection has not been elucidated. Our objective was to assess the effect of HRV-C on cytokine secretion in human bronchial epithelial (HBE) cells grown at air-liquid interface (ALI)...

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Bibliographic Details
Published in:Virology journal 2022-05, Vol.19 (1), p.81-81, Article 81
Main Authors: Yuan, Xin-Hui, Pang, Li-Li, Yang, Jing, Jin, Yu
Format: Article
Language:English
Subjects:
Air–liquid interface
Antibodies
Asthma
Cell culture
Cell differentiation
Chemokine CXCL10
Chemokines
Clinical isolates
Cloning
Cytokines
Enterovirus
Epithelial Cells
Human bronchial epithelial cell
Human rhinovirus C
Humans
Immune response
Immunity
Infections
Interferon
Interleukin 6
Interleukin 8
Monocyte chemoattractant protein 1
Pathogenesis
Plasmids
Pneumonia
Propagation
Proteins
Respiratory syncytial virus
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Human
Rhinovirus
Statistical analysis
Viruses
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Summary:Human rhinovirus C (HRV-C) accounts for a large proportion of HRV-related illnesses, but the immune response to HRV-C infection has not been elucidated. Our objective was to assess the effect of HRV-C on cytokine secretion in human bronchial epithelial (HBE) cells grown at air-liquid interface (ALI) and compare it with that of respiratory syncytial virus (RSV). HBE cells were differentiated at ALI culture and the full-length cDNA clones of HRV-C651 and HRV-C15, clinical isolates of HRV-C79 and HRV-C101, and two RSV isolates were inoculated in the HBE cells. The effect of HRV-C on cytokine secretion was assessed and compared with that of RSV. HRV-Cs infect and propagate in fully differentiated HBE cells and significantly increase the secretion of IFN-λ1, CCL5, IP10, IL-6, IL-8, and MCP-1. The virus loads positively correlated with the levels of the cytokines. HRV-C induced lower secretion of CCL5 (P = 0.048), IL-6 (P = 0.016), MCP-1 (P = 0.008), and IL-8 (P = 0.032), and similar secretion of IP10 (P = 0.214) and IFN-λ1 (P = 0.214) when compared with RSV. HBE ALI culture system supported HRV-C infection and propagation and HRV-C induced relatively weaker cytokine expression than RSV.
ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-022-01805-2