Curcumin Administration Improves Force of mdx Dystrophic Diaphragm by Acting on Fiber-Type Composition, Myosin Nitrotyrosination and SERCA1 Protein Levels

The vegetal polyphenol curcumin displays beneficial effects against skeletal muscle derangement induced by oxidative stress, disuse or aging. Since oxidative stress and inflammation are involved in the progression of muscle dystrophy, the effects of curcumin administration were investigated in the d...

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Bibliographic Details
Published in:Antioxidants 2023-05, Vol.12 (6), p.1181
Main Authors: Gorza, Luisa, Germinario, Elena, Vitadello, Maurizio, Guerra, Irene, De Majo, Federica, Gasparella, Francesca, Caliceti, Paolo, Vitiello, Libero, Danieli-Betto, Daniela
Format: Article
Language:English
Subjects:
Antibodies
Cell cycle
Cloning
Curcumin
Diaphragm
Diaphragm (Anatomy)
Dystrophy
Enzymes
Fibrosis
Inflammation
Kinases
Laboratories
Muscle contraction
muscle dystrophy
Myosin
Myotubes
Nitric oxide
nitrosative stress
nitrotyrosine
nNOS
Oxidative stress
Polyphenols
Proteins
Satellite cells
Skeletal muscle
Tropomyosin
Vehicles
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Summary:The vegetal polyphenol curcumin displays beneficial effects against skeletal muscle derangement induced by oxidative stress, disuse or aging. Since oxidative stress and inflammation are involved in the progression of muscle dystrophy, the effects of curcumin administration were investigated in the diaphragm of mice injected intraperitoneally or subcutaneously with curcumin for 4-12-24 weeks. Curcumin treatment independently of the way and duration of administration (i) ameliorated myofiber maturation index without affecting myofiber necrosis, inflammation and degree of fibrosis; (ii) counteracted the decrease in type 2X and 2B fiber percentage; (iii) increased about 30% both twitch and tetanic tensions of diaphragm strips; (iv) reduced myosin nitrotyrosination and tropomyosin oxidation; (v) acted on two opposite nNOS regulators by decreasing active AMP-Kinase and increasing SERCA1 protein levels, the latter effect being detectable also in myotube cultures from satellite cells. Interestingly, increased contractility, decreased myosin nitrotyrosination and SERCA1 upregulation were also detectable in the diaphragm after a 4-week administration of the NOS inhibitor 7-Nitroindazole, and were not improved further by a combined treatment. In conclusion, curcumin has beneficial effects on the dystrophic muscle, mechanistically acting for the containment of a deregulated nNOS activity.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox12061181