MicroRNA‐324‐5p affects the radiotherapy response of cervical cancer via targeting ELAV‐like RNA binding protein 1

Cervical cancer (CC) seriously threatens the health of women. Radiation therapy (RT) is the major treatment for CC. However, the recurrent CC can acquire resistance to RT. Thus, it is necessary to find a new method for reversing RT resistance in CC. It has been reported that miR‐324‐5p can suppress...

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Bibliographic Details
Published in:The Kaohsiung journal of medical sciences 2020-12, Vol.36 (12), p.965-972
Main Authors: Fan, Ming‐Jun, He, Peng‐Juan, Lin, Xue‐Yan, Yang, Chun‐Run, Li, Chang‐Zhong, Xing, Li‐Gang
Format: Article
Language:English
Subjects:
Apoptosis
Binding sites
Cancer therapies
Cell adhesion & migration
Cells
Cervical cancer
Cloning
ELAVL1
Genetic aspects
Invoices
MicroRNA
MicroRNAs
miR‐324‐5p
Protein binding
Proteins
Radiation therapy
Radiotherapy
Reagents
Scientific equipment and supplies industry
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Summary:Cervical cancer (CC) seriously threatens the health of women. Radiation therapy (RT) is the major treatment for CC. However, the recurrent CC can acquire resistance to RT. Thus, it is necessary to find a new method for reversing RT resistance in CC. It has been reported that miR‐324‐5p can suppress the progression of multiple cancers. However, whether it can reverse resistance to RT in CC remains unclear. qRT‐PCR and Western blotting were used to detect gene and protein expression in CC cells, respectively. Cell proliferation was tested by CCK‐8 assay and colony formation assay. In addition, cell apoptosis was detected by flow cytometry. Transwell assays were performed to detect cell migration. Dual luciferase reporter assay and TargetScan were used to explore the targets of microRNA‐324‐5p (miR‐324‐5p). MiR‐324‐5p was downregulated in CC cells. Overexpression of miR‐324‐5p sensitized CC cells to RT. In addition, miR‐324‐5p mimics significantly induced apoptosis and inhibits the migration of CC cells in the presence of 137Cs ionizing radiation. Furthermore, miR‐324‐5p sensitized CC cells to ionizing radiation by targeting ELAV‐like RNA binding protein 1 (ELAVL1). MiR‐324‐5p overexpression affects the radiotherapy response of CC by targeting ELAVL1, which may serve as a new target for the treatment of CC.
ISSN:1607-551X
2410-8650
DOI:10.1002/kjm2.12277