MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells

• Published in: Journal of experimental & clinical cancer research 2012-06, Vol.31 (1), p.58-58, Article 58
• Main Authors: Wang, Chen, Zheng, Xiangqian, Shen, Chunyan, Shi, Yurong
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing - biosynthesis; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; baculoviral IAP repeat-containing protein 5, Lim and SH3 domain protein 1, Proliferation, Migration; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell adhesion & migration; Cell culture; Cell Growth Processes - genetics; Cell Line, Tumor; Cell Movement - genetics; Cell proliferation; Cytoskeletal Proteins - biosynthesis; Cytoskeletal Proteins - genetics; Cytoskeletal Proteins - metabolism; Development and progression; Down-Regulation; Experiments; Female; Gene Expression Regulation, Neoplastic; Genetic aspects; Humans; Inhibitor of Apoptosis Proteins - biosynthesis; Inhibitor of Apoptosis Proteins - genetics; Inhibitor of Apoptosis Proteins - metabolism; LIM Domain Proteins - biosynthesis; LIM Domain Proteins - genetics; LIM Domain Proteins - metabolism; Luciferase; Medical research; Medicine, Experimental; MicroRNA; MicroRNAs - biosynthesis; MicroRNAs - genetics; MicroRNAs - metabolism; Physiological aspects; Proteins; Receptor, ErbB-2 - metabolism; Receptors, Estrogen - metabolism; Receptors, Progesterone - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - administration & dosage; RNA, Small Interfering - genetics; Transfection; Triple-negative breast cancer, MiR-203
• ISSN: 1756-9966; 0392-9078; 1756-9966
• DOI: 10.1186/1756-9966-31-58

This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC). Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets. Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor. These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.