Properties of Potential Plant-Growth-Promoting Bacteria and Their Effect on Wheat Growth Promotion (Triticum aestivum) and Soil Characteristics

• Published in: Microbiology research 2023-12, Vol.15 (1), p.20-32
• Main Authors: Voronina, Elena, Sokolova, Ekaterina, Tromenschleger, Irina, Mishukova, Olga, Hlistun, Inna, Miroshnik, Matvei, Savenkov, Oleg, Buyanova, Maria, Ivanov, Ilya, Galyamova, Maria, Smirnova, Natalya
• Format: Article
• Language: English
• Subjects: Acetic acid; Ammonia; Ammonium; Antifungal activity; Antimicrobial activity; Bacteria; Biofertilizers; Chemical composition; Environmental conditions; Fertilizers; Fungicides; Gene amplification; Gene sequencing; IAA (indole-3-acetic acid); Indoleacetic acid; Nitrogen; nitrogen fixation; phosphate solubilization; Phosphates; Plant growth; Plant nutrition; plant-growth-promoting bacteria; Potassium; Principal components analysis; Reagents; Regression analysis; rRNA 16S; Seeds; Sequence analysis; siderophore production; Siderophores; Soil bacteria; Soil characteristics; Soil microorganisms; Strains (organisms); Triticum aestivum; Wheat
• ISSN: 2036-7481; 2036-7481
• DOI: 10.3390/microbiolres15010002

Plant-growth-promoting bacteria are an important economic and environmental resource as biofertilizers that can stimulate plant growth and improve agricultural yields. In this study, potential plant growth-promoting bacteria were isolated from soil samples collected in Russia. Strains that manifested active growth on a nitrogen-free medium, the Pikovskaya medium (with insoluble phosphates) and CAS (Chrome Azurol S) agar, were selected for the study. All bacterial isolates were identified by 16S rRNA gene sequencing analysis. Seventeen bacterial isolates of different species were purified and quantified for their ability to grow on nitrogen-free media; dissolve phosphate; and produce ammonium, indole-3-acetic acid, siderophores, and antifungal activity. Principal component analysis identified three groups of strains: one with the maximum signs of providing “plant nutrition”; one with signs of “antimicrobial activity”; and a group “without outstanding signs”. All 17 strains were involved in experiments involving growing inoculated wheat seeds (Triticum aestivum) in pots under natural environmental conditions, and were assessed by their effect on the wheat growth and yield as well as on the chemical composition of the soil. For the “plant nutrition” group, regression analysis revealed a connection between indicators of plant growth, ear length, and ammonium accumulation in the soil. However, in other groups, there were also strains showing a positive effect on plant growth, which suggests the necessity of involving additional factors to predict the ability of strains to affect plants when screened in the laboratory.