The Kinase Activity of Hematopoietic Progenitor Kinase 1 Is Essential for the Regulation of T Cell Function

• Published in: Cell reports (Cambridge) 2018-10, Vol.25 (1), p.80-94
• Main Authors: Hernandez, Sairy, Qing, Jing, Thibodeau, Rebecca Hong, Du, Xiangnan, Park, Summer, Lee, Hyang-Mi, Xu, Min, Oh, Soyoung, Navarro, Armando, Roose-Girma, Meron, Newman, Robert J., Warming, Soren, Nannini, Michelle, Sampath, Deepak, Kim, Jeong M., Grogan, Jane L., Mellman, Ira
• Format: Article
• Language: English
• Subjects: Animals; B7-H1 Antigen - antagonists & inhibitors; B7-H1 Antigen - immunology; cancer immunotherapy; CD8-Positive T-Lymphocytes - enzymology; CD8-Positive T-Lymphocytes - immunology; Colonic Neoplasms - immunology; Colonic Neoplasms - therapy; Female; Glioma - immunology; Glioma - therapy; hematopoietic progenitor kinase 1; HPK1; Immunotherapy; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Protein-Serine-Threonine Kinases - antagonists & inhibitors; Protein-Serine-Threonine Kinases - immunology; Protein-Serine-Threonine Kinases - metabolism; Random Allocation; Signal Transduction; T-Lymphocytes - enzymology; T-Lymphocytes - immunology
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2018.09.012

We examined hematopoietic protein kinase 1 (HPK1), whose reliance on scaffold versus kinase functions for negative immune cell regulation is poorly understood and critical to its assessment as a viable drug target. We identify kinase-dependent roles for HPK1 in CD8 T cells that restrict their anti-viral and anti-tumor responses by using HPK1 kinase-dead (HPK1.kd) knockin mice. Loss of HPK1 kinase function enhanced T cell receptor signaling and cytokine secretion in a T-cell-intrinsic manner. In response to chronic lymphocytic choriomeningitis virus (LCMV) infection or tumor challenge, viral clearance and tumor growth inhibition were enhanced in HPK1.kd mice, accompanied by an increase in effector CD8 T cell function. Co-blockade of PD-L1 further enhanced T effector cell function, resulting in superior anti-viral and anti-tumor immunity over single target blockade. These results identify the importance of HPK1 kinase activity in the negative regulation of CD8 effector functions, implicating its potential as a cancer immunotherapy target. [Display omitted] •HPK1 kinase activity limits TCR signaling and effector cytokine secretion•Loss of HPK1 kinase activity enhances anti-viral responses in preclinical models•Loss of HPK1 kinase function suppresses tumor growth in preclinical tumor models•Therapeutic co-blockade of HPK1 kinase and PD-L1 enhances anti-tumor responses HPK1 is implicated in several important steps that limit T cell responsiveness, but the mechanism is poorly characterized. Hernandez et al. demonstrate that HPK1’s kinase activity is essential for attenuating T cell function. Loss of HPK1 kinase activity enhanced antitumoral responses, proving to be an attractive target for cancer immunotherapy.