Advancing PEDV Vaccination: Comparison between Inactivated and Flagellin N-Terminus-Adjuvanted Subunit Vaccines

Vaccinations can serve as an important preventive measure against the porcine epidemic diarrhea (PED) virus that currently threatens the swine industry. This study focuses on the development of a fusion protein vaccine, FliC - COE, which combines the N-terminus of flagellin (FliC ) with a modified c...

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Bibliographic Details
Published in:Vaccines (Basel) 2024-01, Vol.12 (2), p.139
Main Authors: Murtaza, Asad, Hoa, Nguyen-Thanh, Dieu-Huong, Do, Afzal, Haroon, Tariq, Muhammad Hamza, Cheng, Li-Ting, Chung, Yao-Chi
Format: Article
Language:English
Subjects:
adjuvant
Antibodies
Cell proliferation
Cell-mediated immunity
chimeric protein
Deactivation
Diarrhea
Diseases
Dosage and administration
Enzyme-linked immunosorbent assay
Epidemics
Epitopes
Flagellin
Fusion protein
Hogs
Immune system
Immunity
Immunology
Molecular docking
Molecular dynamics
N-Terminus
Neutralization
piglet
porcine epidemic diarrhea virus
Protein expression
Proteins
Simulation
Swine
Testing
Transmissible gastroenteritis
Vaccines
Viral vaccines
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Summary:Vaccinations can serve as an important preventive measure against the porcine epidemic diarrhea (PED) virus that currently threatens the swine industry. This study focuses on the development of a fusion protein vaccine, FliC - COE, which combines the N-terminus of flagellin (FliC ) with a modified core neutralizing epitope ( COE) of PEDV. In silico immunoinformatic analysis confirmed the construct's non-toxic, non-allergenic, and highly antigenic nature. Molecular docking and molecular dynamics (MD) simulations demonstrated FliC - COE's strong binding to the TLR-5 immunological receptor. Repeated exposure simulations and immunological simulations suggested enhanced cell-mediated immunity. Both FliC - COE and an inactivated PEDV vaccine were produced and tested in mice. The results from cell proliferation, ELISA, and neutralization assays indicated that FliC - COE effectively stimulated cellular immunity and neutralized PEDV. We conclude that the FliC - COE fusion protein may serve as a promising vaccine candidate against PEDV.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines12020139