Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma

Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on...

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Bibliographic Details
Published in:Stem cells international 2021-07, Vol.2021, p.6686625-11
Main Authors: Mun, Sue Jean, Kang, Shin Ae, Park, Hye-Kyung, Yu, Hak Sun, Cho, Kyu-Sup, Roh, Hwan-Jung
Format: Article
Language:English
Subjects:
Albumin
Allergens
Allergic diseases
Allergies
Analysis
Asthma
Body fat
Bronchus
Cell culture
Cytokines
Eosinophils
Extracellular vesicles
Immunoglobulin G
Immunoglobulins
Immunomodulation
Inflammation
Interleukins
Lavage
Leukocytes (eosinophilic)
Lungs
Lymph nodes
Lymphatic system
Lymphocytes
Lymphocytes T
Mice
Ovalbumin
Respiratory tract diseases
Stem cell transplantation
Stem cells
T cells
Vesicles
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Summary:Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2021/6686625