Lidocaine and Pinacidil Added to Blood versus Crystalloid Cardioplegic Solutions: Study in Isolated Hearts

• Published in: Revista brasileira de cirurgia cardiovascular 2018-01, Vol.33 (3), p.211-216
• Main Authors: Carmo, Helison Pereira do, Reichert, Karla, Carvalho, Daniela Diógenes de, Silveira-Filho, Lindemberg da Mota, Vilarinho, Karlos, Oliveira, Pedro, Petrucci, Orlando
• Format: Article
• Language: English
• Subjects: Animals; Blotting, Western; CARDIAC & CARDIOVASCULAR SYSTEMS; Cardiology; Cardioplegic Solutions; Cardioplegic Solutions - chemistry; Cardioplegic Solutions - pharmacology; Carrier Proteins - analysis; Clinical medicine; Coronary Vessels - physiopathology; Drug dosages; Glucose - chemistry; Glucose - pharmacology; Heart - drug effects; Heart Arrest, Induced - methods; Heart surgery; Ischemia; Laboratory animals; Lidocaine - pharmacology; Male; Mannitol - chemistry; Mannitol - pharmacology; Metabolism; Microfilament Proteins - analysis; Models, Animal; Myocardial Reperfusion - methods; Myocardial Reperfusion Injury; Myocardial Reperfusion Injury - pathology; Myocardial Reperfusion Injury - prevention & control; Original; Ostomy; Pinacidil - pharmacology; Potassium Chloride - chemistry; Potassium Chloride - pharmacology; Potassium Compounds - chemistry; Potassium Compounds - pharmacology; Procaine - chemistry; Procaine - pharmacology; Rats, Wistar; Reproducibility of Results; SURGERY; Time Factors; Transplants & implants; Vascular Resistance - physiology; Ventricular Function, Left - physiology
• ISSN: 1678-9741; 0102-7638; 1678-9741
• DOI: 10.21470/1678-9741-2017-0244

The present study aimed the functional recovery evaluation after long term of cardiac arrest induced by Custodiol (crystalloid-based) versus del Nido (blood-based) solutions, both added lidocaine and pinacidil as cardioplegic agents. Experiments were performed in isolated rat heart perfusion models. Male rat heart perfusions, according to Langendorff technique, were induced to cause 3 hours of cardiac arrest with a single dose. The hearts were assigned to one of the following three groups: (I) control; (II) Custodiol-LP; and (III) del Nido-LP. They were evaluated after ischemia throughout 90 minutes of reperfusion. Left ventricular contractility function was reported as percentage of recovery, expressed by developed pressure, maximum dP/dt, minimum dP/dt, and rate pressure product variables. In addition, coronary resistance and myocardial injury marker by alpha-fodrin degradation were also evaluated. At 90 minutes of reperfusion, both solutions had superior left ventricular contractile recovery function than the control group. Del Nido-LP was superior to Custodiol-LP in maximum dP/dt (46%±8 vs. 67%±7, P