Plasma mtDNA as a possible contributor to and biomarker of inflammation in rheumatoid arthritis

Neutrophil extracellular trap formation and cell-free DNA (cfDNA) contribute to the inflammation in rheumatoid arthritis (RA), but it is unknown if mitochondrial DNA (mtDNA) or nuclear DNA (nDNA) is more abundant in the circulation. It is unclear if DNA concentration measurements may assist in clini...

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Bibliographic Details
Published in:Arthritis research & therapy 2024-05, Vol.26 (1), p.97-97, Article 97
Main Authors: Lehmann, Julia, Giaglis, Stavros, Kyburz, Diego, Daoudlarian, Douglas, Walker, Ulrich A
Format: Article
Language:English
Subjects:
Adult
Aged
Analysis
Arthritis
Arthritis, Rheumatoid - blood
Arthritis, Rheumatoid - diagnosis
Biomarkers - blood
Blood donors
Care and treatment
Decision-making
Diagnosis
DNA, Mitochondrial - blood
Ethylenediaminetetraacetic acid
Female
Genetic aspects
Health aspects
Humans
Inflammation
Inflammation - blood
Inflammation - diagnosis
Male
Medical research
Medicine, Experimental
Middle Aged
Mitochondrial DNA
Prospective Studies
Rheumatoid arthritis
Rheumatoid factor
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Summary:Neutrophil extracellular trap formation and cell-free DNA (cfDNA) contribute to the inflammation in rheumatoid arthritis (RA), but it is unknown if mitochondrial DNA (mtDNA) or nuclear DNA (nDNA) is more abundant in the circulation. It is unclear if DNA concentration measurements may assist in clinical decision-making. This single-center prospective observational study collected plasma from consecutive RA patients and healthy blood donors. Platelets were removed, and mtDNA and nDNA copy numbers were quantified by polymerase chain reaction (PCR). One hundred six RA patients and 85 healthy controls (HC) were recruited. Circulating median mtDNA copy numbers were increased 19.4-fold in the plasma of patients with RA (median 1.1 x10 copies/mL) compared to HC (median 5.4 x10 copies/mL, p
ISSN:1478-6362
1478-6354
1478-6362
DOI:10.1186/s13075-024-03329-2