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Functional characterization of QT interval associated SCN5A enhancer variants identify combined additive effects

Several empirical and theoretical studies suggest the presence of multiple enhancers per gene that collectively regulate gene expression, and that common sequence variation impacting on the activities of these enhancers is a major source of inter-individual gene expression variability. However, for...

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Bibliographic Details
Published in:HGG advances 2024-09, Vol.6 (1), p.100358, Article 100358
Main Authors: Gunamalai, Lavanya, Singh, Parul, Berg, Brian, Shi, Leilei, Sanchez, Ernesto, Smith, Alexa, Breton, Ghislain, Bedford, Mark T., Balciunas, Darius, Kapoor, Ashish
Format: Article
Language:English
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Summary:Several empirical and theoretical studies suggest the presence of multiple enhancers per gene that collectively regulate gene expression, and that common sequence variation impacting on the activities of these enhancers is a major source of inter-individual gene expression variability. However, for the vast majority of genes, enhancers and the underlying regulatory variation remains unknown. Even for the genes with well-characterized enhancers, the nature of the combined effects from multiple enhancers and their variants, when known, on gene expression regulation remains unexplored. Here, we have evaluated the combined effects from five SCN5A enhancers and their regulatory variants that are known to collectively correlate with SCN5A cardiac expression and underlie QT interval association in the general population. Using small deletions centered at the regulatory variants in episomal reporter assays in a mouse cardiomyocyte cell line, we demonstrate that the variants and their flanking sequences play critical role in individual enhancer activities, likely being a transcription factor (TF) binding site. By oligonucleotide-based pulldown assays on predicted TFs, we identify the TFs likely driving allele-specific enhancer activities. Using all 32 possible allelic synthetic constructs in reporter assays, representing the five bi-allelic enhancers, we demonstrate combined additive effects on overall enhancer activities. Using transient enhancer assays in zebrafish embryos we demonstrate that four elements act as enhancers in vivo. Together, these studies uncover the TFs driving the enhancer activities of QT interval associated SCN5A regulatory variants, reveal the additive effects from allelic combinations of these regulatory variants, and prove their potential to act as enhancers in vivo. Multiple enhancers and their sequence variants are expected to collectively regulate gene expression. However, functional evidence supportive of their combinatorial effects are lacking. Here, for five SCN5A enhancer variants, we identify their combined additive effects on reporter expression, their cognate TFs, and confirm their enhancer activities in vivo.
ISSN:2666-2477
2666-2477
DOI:10.1016/j.xhgg.2024.100358