Genetically Encoded Fluorescent Biosensors for Biomedical Applications

One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropri...

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Bibliographic Details
Published in:Biomedicines 2021-10, Vol.9 (11), p.1528
Main Authors: Ovechkina, Vera S., Zakian, Suren M., Medvedev, Sergey P., Valetdinova, Kamila R.
Format: Article
Language:English
Subjects:
Bioavailability
Bioluminescence
Biosensors
Cytotoxicity
Drug screening
fluorescent protein
genetically encoded fluorescent biosensors
Kinases
Laboratories
Ligands
Localization
Physiology
Proteins
Review
Sensors
Visualization
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Summary:One of the challenges of modern biology and medicine is to visualize biomolecules in their natural environment, in real-time and in a non-invasive fashion, so as to gain insight into their physiological behavior and highlight alterations in pathological settings, which will enable to devise appropriate therapeutic strategies. Genetically encoded fluorescent biosensors constitute a class of imaging agents that enable visualization of biological processes and events directly in situ, preserving the native biological context and providing detailed insight into their localization and dynamics in cells. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to screen at the single-cell resolution; and the elimination of false-positive results caused by low drug bioavailability that is not detected by in vitro testing methods are a few of the obvious benefits of using genetically encoded fluorescent biosensors in drug screening. This review summarizes results of the studies that have been conducted in the last years toward the fabrication of genetically encoded fluorescent biosensors for biomedical applications with a comprehensive discussion on the challenges, future trends, and potential inputs needed for improving them.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9111528