A convenient method for lecithin purification from fresh eggs

The increasing demand for fatty acid-free lecithin required modifications in existing purification methods. In this technical note we describe a purification procedure with the following steps: a) homogenization and extraction of yolks obtained from fresh eggs with acetone, b) solubilization with et...

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Published in:Química Nova 2008, Vol.31 (4), p.910-913
Main Authors: Maximiano, Flavio A., Silva, Marcia A. da, Daghastanli, Katia R. P., Araujo, Pedro S. de, Chaimovich, Hernan, Cuccovia, Iolanda M.
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Language:English
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CHEMISTRY, MULTIDISCIPLINARY
lecithin
phosphatidylcholine
purification
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container_issue 4
container_start_page 910
container_title Química Nova
container_volume 31
creator Maximiano, Flavio A.
Silva, Marcia A. da
Daghastanli, Katia R. P.
Araujo, Pedro S. de
Chaimovich, Hernan
Cuccovia, Iolanda M.
description The increasing demand for fatty acid-free lecithin required modifications in existing purification methods. In this technical note we describe a purification procedure with the following steps: a) homogenization and extraction of yolks obtained from fresh eggs with acetone, b) solubilization with ethanol and solvent elimination and c) repeated solubilization/precipitation with petroleum ether/acetone. This crude extract was chromatographed on neutral alumina, which was exhaustively washed with chloroform before elution with chloroform:methanol, allowing the sequential separation of fatty acids and lecithin. Chromatographic behavior and mass spectra of the product are presented. This fast procedure yields fatty acid-free lecithin at a competitive cost.
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subjects CHEMISTRY, MULTIDISCIPLINARY
lecithin
phosphatidylcholine
purification
title A convenient method for lecithin purification from fresh eggs
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