Clever Experimental Designs: Shortcuts for Better iPSC Differentiation

For practical use of pluripotent stem cells (PSCs) for disease modelling, drug screening, and regenerative medicine, the cell differentiation process needs to be properly refined to generate end products with consistent and high quality. To construct and optimize a robust cell-induction process, a m...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-12, Vol.10 (12), p.3540
Main Authors: Yasui, Ryota, Sekine, Keisuke, Taniguchi, Hideki
Format: Article
Language:English
Subjects:
Animals
Arrays
Cell culture
Cell Differentiation
Cell division
Cell Proliferation
Chinese hamster ovary (CHO) cell
Decision Trees
Design of experiments
design of experiments (DOE)
Drug screening
Efficiency
embryonic stem cell (ESC)
hematopoietic stem cell (HSC)
Hematopoietic stem cells
Humans
induced pluripotent stem cell (iPSC)
Induced Pluripotent Stem Cells - cytology
Industrial applications
Medical research
mesenchymal stem cell (MSC)
Mesenchyme
Optimization
Organoids
Pluripotency
Product development
Regenerative medicine
Reproducibility
Research Design
Research methodology
Review
Stem cell transplantation
Stem cells
Variance analysis
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Summary:For practical use of pluripotent stem cells (PSCs) for disease modelling, drug screening, and regenerative medicine, the cell differentiation process needs to be properly refined to generate end products with consistent and high quality. To construct and optimize a robust cell-induction process, a myriad of cell culture conditions should be considered. In contrast to inefficient brute-force screening, statistical design of experiments (DOE) approaches, such as factorial design, orthogonal array design, response surface methodology (RSM), definitive screening design (DSD), and mixture design, enable efficient and strategic screening of conditions in smaller experimental runs through multifactorial screening and/or quantitative modeling. Although DOE has become routinely utilized in the bioengineering and pharmaceutical fields, the imminent need of more detailed cell-lineage specification, complex organoid construction, and a stable supply of qualified cell-derived material requires expedition of DOE utilization in stem cell bioprocessing. This review summarizes DOE-based cell culture optimizations of PSCs, mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and Chinese hamster ovary (CHO) cells, which guide effective research and development of PSC-derived materials for academic and industrial applications.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells10123540