Optimization of supercritical fluid extraction and HPLC identification of wedelolactone from Wedelia calendulacea by orthogonal array design

The purpose of this work is to provide a complete study of the influence of operational parameters of the supercritical carbon dioxide assisted extraction (SC CO2E) on yield of wedelolactone from Wedelia calendulacea Less., and to find an optimal combination of factors that maximize the wedelolacton...

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Bibliographic Details
Published in:Journal of advanced research 2014-11, Vol.5 (6), p.629-635
Main Authors: Patil, Ajit A., Sachin, Bhusari S., Wakte, Pravin S., Shinde, Devanand B.
Format: Article
Language:English
Subjects:
High-performance liquid chromatography
Original
Supercritical carbon dioxide extraction
Taguchi orthogonal array design
Wedelia calendulacea
Wedelolactone
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Summary:The purpose of this work is to provide a complete study of the influence of operational parameters of the supercritical carbon dioxide assisted extraction (SC CO2E) on yield of wedelolactone from Wedelia calendulacea Less., and to find an optimal combination of factors that maximize the wedelolactone yield. In order to determine the optimal combination of the four factors viz. operating pressure, temperature, modifier concentration and extraction time, a Taguchi experimental design approach was used: four variables (three levels) in L9 orthogonal array. Wedelolactone content was determined using validated HPLC methodology. Optimum extraction conditions were found to be as follows: extraction pressure, 25MPa; temperature, 40°C; modifier concentration, 10% and extraction time, 90min. Optimum extraction conditions demonstrated wedelolactone yield of 8.01±0.34mg/100g W. calendulacea Less. Pressure, temperature and time showed significant (p
ISSN:2090-1232
2090-1224
DOI:10.1016/j.jare.2013.09.002