Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection

Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospital...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2008-03, Vol.103 (2), p.195-200
Main Authors: Rosete, Dora P, Manjarrez, María Eugenia, Barrón, Blanca L
Format: Article
Language:English
Subjects:
Acute Disease
adenovirus C
Adenovirus Infections, Human - diagnosis
Adenovirus Infections, Human - epidemiology
Adenovirus Infections, Human - virology
Adenoviruses, Human - genetics
Adenoviruses, Human - isolation & purification
ARI
Bacterial Typing Techniques
Child
DNA Restriction Enzymes - analysis
Female
Genetic Markers
Genome, Viral
Humans
Male
Mexico - epidemiology
Nasopharynx - virology
non-hospitalized children
PARASITOLOGY
PCR/RFLP
PCR/RFLP - adenovirus C - ARI - non-hospitalized children
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Respiratory Tract Infections - diagnosis
Respiratory Tract Infections - epidemiology
Respiratory Tract Infections - virology
Seasons
TROPICAL MEDICINE
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Summary:Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23%, and only AdV C was detected.
ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02762008000200012