Plasmon resonance biosensor for interleukin-1β point-of-care determination: A tool for early periodontitis diagnosis

Among pro-inflammatory cytokines, Interleukin-1β is crucially involved in several inflammatory-based diseases and even cancer. Increased Interleukin-1β levels in oral fluids have been proposed as an early marker of periodontitis, a broadly diffused chronic inflammatory condition of periodontal-suppo...

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Published in:iScience 2024-01, Vol.27 (1), p.108741, Article 108741
Main Authors: Cennamo, Nunzio, Bencivenga, Debora, Annunziata, Marco, Arcadio, Francesco, Stampone, Emanuela, Piccirillo, Angelantonio, Della Ragione, Fulvio, Zeni, Luigi, Guida, Luigi, Borriello, Adriana
Format: Article
Language:English
Subjects:
Biological sciences
Health technology
Sensor
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Summary:Among pro-inflammatory cytokines, Interleukin-1β is crucially involved in several inflammatory-based diseases and even cancer. Increased Interleukin-1β levels in oral fluids have been proposed as an early marker of periodontitis, a broadly diffused chronic inflammatory condition of periodontal-supporting tissues, leading eventually to tooth loss. We describe the development of a portable surface-plasmon-resonance-based optical fiber probe suitably coated with an anti-Interleukin-1β antibody monolayer. A pico-nanomolar linear range of determination was obtained in both buffer solution and saliva with a rapid (3 min) incubation and high selectivity in presence of interferents. Higher Interleukin-1β concentration in the saliva of a periodontitis patient compared to a healthy control was determined. These measurements were validated by an automated ELISA system. Our results sustain the potential applicability of the proposed SPR-POF as diagnostic point-of-care device for real-time monitoring of salivary Interleukin-1β, that can support early detection of oral inflammatory-based pathologies and rapid and timely therapeutic decisions. [Display omitted] •SPR and antibody-embedded surface are used to develop a portable Il-1β biosensor•LoD in the picomolar range, short time of analysis, high selectivity are demonstrated•Clinically relevant Il-1β concentration can be detected in complex real matrices•The developed biosensor can support early detection of oral inflammatory pathologies Health technology; Sensor; Biological sciences
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2023.108741