Diagnostic Accuracy of Direct Immunofluorescence Test on Paraffin-Embedded Blocks in Comparison with Frozen Section Blocks in Renal Biopsies

Background. In several published research, the evaluation of renal disorders using immunofluorescence on formalin-fixed, paraffin-embedded (FFPE) tissue sections versus immunofluorescence on frozen sections was compared. Each technique’s accuracy varies greatly. This study’s objective was to assess...

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Bibliographic Details
Published in:International journal of nephrology 2022-10, Vol.2022, p.1-10
Main Authors: Mohammadzadeh, Sahand, Aghakhaninejad, Fatemeh, Azad, Fariborz, Derakhshan, Dorna, Soleimani, Neda
Format: Article
Language:English
Subjects:
Alcohol
Antibodies
Antigens
Biopsy
Comparative analysis
Ethylenediaminetetraacetic acid
Fluorescent antibody technique
Formaldehyde
Glycerol
Immunofluorescence
Immunoglobulin A
Immunoglobulin G
Investigations
Lupus
Medical research
Medicine, Experimental
Methods
Microscopy
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Summary:Background. In several published research, the evaluation of renal disorders using immunofluorescence on formalin-fixed, paraffin-embedded (FFPE) tissue sections versus immunofluorescence on frozen sections was compared. Each technique’s accuracy varies greatly. This study’s objective was to assess IF-P as a potential replacement for IF-F in the diagnosis of renal biopsy specimens. Materials and Methods. To show immunoglobulin IgA, IgG, IgM, and C3 immune deposits, proteinase K digestion of paraffin-embedded renal biopsy was standardized and used in 51 renal biopsies. Sensitivity, specificity, false-positive, and false-negative values were calculated. Results. IF-P showed a sensitivity of 93.1%, 76.9%, 63.6%, and 33.3%, and a specificity of 100%, 97.3%, 95%, and 100% for IgG, IgA, IgM, and C3, respectively. Compared to cases that had both routine IF and IF-P, 50 of 51 showed either the same amount of staining for the diagnostic immunoglobulin/complement or a small amount of difference. In most of the cases (49 of 51), diagnostic findings were found. Conclusion. IF-P is a sensitive and precise approach for assessing immune deposits in renal tissue biopsies. We come to the conclusion that IF-P serves as a beneficial salvage immunohistochemistry method for renal biopsies that do not contain enough cortical tissue for IF-F.
ISSN:2090-214X
2090-2158
2090-2158
DOI:10.1155/2022/4974031