Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis

The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profil...

Full description

Saved in:
Bibliographic Details
Published in:Biological research 2010, Vol.43 (1), p.51-62
Main Authors: Sant'Anna, Juliane R, Miyamoto, Cláudia T, Rosada, Lúcia J, Franco, Claudinéia C S, Kaneshima, Edilson N, Castro-Prado, Marialba A A
Format: Article
Language:English
Subjects:
BIOLOGY
Brazil
Colletotrichum - classification
Colletotrichum - genetics
Colletotrichum - isolation & purification
genetic variability
Genetic Variation - genetics
Genotype
Glycine max - microbiology
heterokaryosis
Mutation - genetics
parasexual recombination
Random Amplified Polymorphic DNA Technique
RAPD
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and their nit1 and NitM mutants. RAPD amplification, with five different primers, also showed polymorphic profiles among Brazilian C. truncatum isolates. Dendrogram analysis resulted in a similarity degree ranging between 0.331 and 0.882 among isolates and identified three RAPD groups. Despite the lack of a correlation between the RAPD analysis and the vegetative compatibility grouping, results demonstrated the potential of VCG analysis to differentiate C. truncatum isolates genotypically similar when compared by RAPD.
ISSN:0716-9760
0717-6287
DOI:10.4067/S0716-97602010000100007