Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma brucei

The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of t...

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Bibliographic Details
Published in:iScience 2020-12, Vol.23 (12), p.101780, Article 101780
Main Authors: Rajan, K. Shanmugha, Doniger, Tirza, Cohen-Chalamish, Smadar, Rengaraj, Praveenkumar, Galili, Beathrice, Aryal, Saurav, Unger, Ron, Tschudi, Christian, Michaeli, Shulamit
Format: Article
Language:English
Subjects:
Molecular Biology
Omics
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Summary:The parasite Trypanosoma brucei is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of trans-splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact in vivo with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2020.101780