Transcriptomic analysis of nonylphenol effect on Saccharomyces cerevisiae

Nonylphenol (NP) is a bioaccumulative environmental estrogen that is widely used as a nonionic surfactant. We have previously examined short-term effects of NP on yeast cells using microarray technology. In the present study, we investigated the adaptive response of BY4742 cells to NP exposure by an...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2021-02, Vol.9, p.e10794-e10794, Article e10794
Main Authors: Bereketoglu, Ceyhun, Nacar, Gozde, Sari, Tugba, Mertoglu, Bulent, Pradhan, Ajay
Format: Article
Language:English
Subjects:
Autophagy
Biosynthesis
Breastfeeding & lactation
Cell cycle
Cell cycle process
Deoxyribonucleic acid
DNA
DNA microarrays
DNA repair
Drug resistance
Ecotoxicology
Estrogens
Ethanol
Gene expression
Genetic engineering
Genomes
Genomics
Glucose
Microbiology
Mismatch repair
Molecular Biology
Molekylärbiologi
Mycology
Nonylphenol
Oxidative phosphorylation
OXPHOS
Phagocytosis
Phosphorylation
Pollutants
Ribonucleic acid
RNA
RNA-seq
Saccharomyces cerevisiae
Toxicology
Transcription
Yeast
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Summary:Nonylphenol (NP) is a bioaccumulative environmental estrogen that is widely used as a nonionic surfactant. We have previously examined short-term effects of NP on yeast cells using microarray technology. In the present study, we investigated the adaptive response of BY4742 cells to NP exposure by analyzing genome-wide transcriptional profiles using RNA-sequencing. We used 2 mg/L NP concentration for 40 days of exposure. Gene expression analysis showed that a total of 948 genes were differentially expressed. Of these, 834 genes were downregulated, while 114 genes were significantly upregulated. GO enrichment analysis revealed that 369 GO terms were significantly affected by NP exposure. Further analysis showed that many of the differentially expressed genes were associated with oxidative phosphorylation, iron and copper acquisition, autophagy, pleiotropic drug resistance and cell cycle progression related processes such as DNA and mismatch repair, chromosome segregation, spindle checkpoint activity, and kinetochore organization. Overall, these results provide considerable information and a comprehensive understanding of the adaptive response to NP exposure at the gene expression level.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.10794