Osseointegration of layer-by-layer polyelectrolyte multilayers loaded with IGF1 and coated on titanium implant under osteoporotic condition

• Published in: International journal of nanomedicine 2017-01, Vol.12, p.7709-7720
• Main Authors: Xing, Helin, Wang, Xing, Xiao, Saisong, Zhang, Guilan, Li, Meng, Wang, Peihuan, Shi, Quan, Qiao, Pengyan, E, Lingling, Liu, Hongchen
• Format: Article
• Language: English
• Subjects: Contact angle; Hospitals; IGF1; Insulin; Insulin-like growth factors; layer-by-layer; Mammals; Metabolism; Methods; Original Research; Osseointegration; Osteoporosis; polyelectrolyte multilayers; Titanium alloys; titanium implant; Transplants & implants
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S148001

Titanium implant is a widely used method for dental prosthesis restoration. Nevertheless, in patients with systemic diseases, including osteoporosis, diabetes, and cancer, the success rate of the implant is greatly reduced. This study investigates a new implant material loaded with insulin-like growth factor 1 (IGF1), which could potentially improve the implant success rate, accelerate the occurrence of osseointegration, and provide a new strategy for implant treatment in osteoporotic patients. Biofunctionalized polyelectrolyte multilayers (PEMs) with polyethylenimine as the excitation layer and gelatin/chitosan loaded with IGF1 were prepared on the surface of titanium implant by layer-by-layer self-assembly technique. The physical and chemical properties of the biofunctionalized PEMs, the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), and bone implant contact correlation test indexes were detected and analyzed in vitro and in vivo using osteoporosis rat model. PEMs coatings loaded with IGF1 (TNS-PEM-IGF1-100) implant promoted the early stage of BMMSCs adhesion. Under the action of body fluids, the active coating showed sustained release of growth factors, which in turn promoted the proliferation and differentiation of BMMSCs and the extracellular matrix. At 8 weeks from implant surgery, the new bone around the implants was examined using micro-CT and acid fuchsin/methylene blue staining. The new bone formation increased with time in each group, while the TNS-PEM-IGF1-100 group showed the highest thickness and continuity. TNS-PEM-IGF1-100 new implants can promote osseointegration in osteoporotic conditions both in vivo and in vitro and provide a new strategy for implant repair in osteoporotic patients.