Ion Channel and Ubiquitin Differential Expression during Erythromycin-Induced Anhidrosis in Foals

Macrolide drugs are the treatment of choice for infections, despite severe side-effects temporary anhidrosis as a. To better understand the molecular biology leading to macrolide induced anhidrosis, we performed skin biopsies and Quantitative Intradermal Terbutaline Sweat Tests (QITSTs) in six healt...

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Bibliographic Details
Published in:Animals (Basel) 2021-11, Vol.11 (12), p.3379
Main Authors: Patterson Rosa, Laura, Mallicote, Martha F, MacKay, Robert J, Brooks, Samantha A
Format: Article
Language:English
Subjects:
Biology
Biopsy
channelopathy
chronic idiopathic anhidrosis
Drugs
Erythromycin
Fever
Gene expression
Genes
Heat
Humidity
Hyperthermia
Infections
Juveniles
Molecular biology
non-sweater
Pharmacy
Pneumonia
rhodococcosis
Rhodococcus equi
RNA-seq
Side effects
Skin
Sweat
Sweat gland
Sweating
Terbutaline
transcriptomics
Ubiquitin
Ubiquitination
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Summary:Macrolide drugs are the treatment of choice for infections, despite severe side-effects temporary anhidrosis as a. To better understand the molecular biology leading to macrolide induced anhidrosis, we performed skin biopsies and Quantitative Intradermal Terbutaline Sweat Tests (QITSTs) in six healthy pony-cross foals for three different timepoints during erythromycin administration-pre-treatment (baseline), during anhidrosis and post-recovery. RNA sequencing of biopsies followed by differential gene expression analysis compared both pre and post normal sweating timepoints to the erythromycin induced anhidrosis episode. After Bonferroni correction for multiple testing, 132 gene transcripts were significantly differentially expressed during the anhidrotic timepoint. Gene ontology analysis of the full differentially expressed gene set identified over-represented biological functions for ubiquitination and ion-channel function, both biologically relevant to sweat production. These same mechanisms were previously implicated in heritable equine idiopathic anhidrosis and sweat gland function and their involvement in macrolide-induced temporary anhidrosis warrants further investigation.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11123379