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Role of hsa_circ_0007482 in pterygium development: insights into proliferation, apoptosis, and clinical correlations
To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts (HPFs) and its correlation with the severity grades of pterygium. Pterygium and normal conjunctival tissues were collected from the superior area of the same patient's eye ( =33). The...
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Published in: | International journal of ophthalmology 2024-08, Vol.17 (8), p.1387-1395 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts (HPFs) and its correlation with the severity grades of pterygium.
Pterygium and normal conjunctival tissues were collected from the superior area of the same patient's eye (
=33). The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reverse-transcription polymerase chain reaction (RT-qPCR) were analyzed. Three distinct siRNA sequences targeting hsa_circ_0007482, along with a negative control sequence, were transfected into HPFs. Cell proliferation was assessed using the cell counting kit-8. Expression levels of Ki67, proliferating cell nuclear antigen (PCNA), Cyclin D1, Bax, B-cell lymphoma-2 (Bcl-2), and Caspase-3 were measured
RT-qPCR. Immunofluorescence staining was employed to detect Ki67 and vimentin expressions. Apoptosis was evaluated using flow cytometry.
Hsa_circ_0007482 expression was significantly higher in pterygium tissues compared to normal conjunctival tissues ( |
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ISSN: | 2222-3959 2227-4898 |
DOI: | 10.18240/ijo.2024.08.01 |