Structural characterization of the degradation products of a minor natural sweet diterpene glycoside Rebaudioside M under acidic conditions

Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental resu...

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Bibliographic Details
Published in:International journal of molecular sciences 2014-01, Vol.15 (1), p.1014-1025
Main Authors: Prakash, Indra, Chaturvedula, Venkata Sai Prakash, Markosyan, Avetik
Format: Article
Language:English
Subjects:
Acids - chemistry
degradation products
Diterpenes, Kaurane - chemistry
Diterpenes, Kaurane - pharmacology
Hydrogen-Ion Concentration
Hydrolysis
rebaudioside M
spectroscopic data
stability
structure characterization
Sweetening Agents - chemistry
Temperature
Trisaccharides - chemistry
Trisaccharides - pharmacology
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Summary:Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) & 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms15011014