Development of a Multicomponent Microbiological Soil Inoculant and Its Performance in Sweet Potato Cultivation

• Published in: Microorganisms (Basel) 2023-03, Vol.11 (4), p.914
• Main Authors: Nagy, Viktor Dávid, Zhumakayev, Anuar, Vörös, Mónika, Bordé, Ádám, Szarvas, Adrienn, Szűcs, Attila, Kocsubé, Sándor, Jakab, Péter, Monostori, Tamás, Škrbić, Biljana D, Mohai, Edina, Hatvani, Lóránt, Vágvölgyi, Csaba, Kredics, László
• Format: Article
• Language: English
• Subjects: Acetic acid; Agricultural land; Agriculture; Agrochemicals; Air pollution; Arthrobacter; Bacillus; biocontrol; Biodegradation; Biological control; Consortia; Cultivation; Enzymatic activity; Enzymes; Equipment and supplies; Field tests; Fungi; Fungicides; Glucose; Indoleacetic acid; Ipomoea batatas; Management; Metabolites; Methods; microbiological soil inoculant; Microorganisms; Nitrogen; Nitrogen fixation; Nitrogenation; Nutrients; Pathogens; Pest control; Pesticides; Pests; Plant growth; Potatoes; Pseudomonas; RNA polymerase; Soil amendments; Soil inoculation; Soil microorganisms; Soil pollution; Soil water; Soils; Strains (organisms); Survivability; Sweet potatoes; Temperature tolerance; Testing; Trichoderma; Vegetables; Water activity; Water pollution
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11040914

The cultivation and consumption of sweet potato ( ) are increasing globally. As the usage of chemical fertilizers and pest control agents during its cultivation may lead to soil, water and air pollution, there is an emerging need for environment-friendly, biological solutions enabling increased amounts of healthy crop and efficient disease management. Microbiological agents for agricultural purposes gained increasing importance in the past few decades. Our goal was to develop an agricultural soil inoculant from multiple microorganisms and test its application potential in sweet potato cultivation. Two strains were selected: strain SZMC 25217 based on its extracellular enzyme activities for the biodegradation of plant residues, and strain SZMC 25231 for biocontrol purposes against fungal plant pathogens. The strain SZMC 24986 proved to be the best growth inhibitor of most of the nine tested strains of fungal species known as plant pathogens, therefore it was also selected for biocontrol purposes against fungal plant pathogens. strain SZMC 25081, showing the fastest growth on nitrogen-free medium, was selected as a component with possible nitrogen-fixing potential. A strain, SZMC 25872, was selected for its ability to produce indole-3-acetic acid, which is among the important traits of potential plant growth-promoting rhizobacteria (PGPR). A series of experiments were performed to test the selected strains for their tolerance to abiotic stress factors such as pH, temperature, water activity and fungicides, influencing the survivability in agricultural environments. The selected strains were used to treat sweet potato in two separate field experiments. Yield increase was observed for the plants treated with the selected microbial consortium (synthetic community) in comparison with the control group in both cases. Our results suggest that the developed microbial inoculant has the potential to be used in sweet potato plantations. To the best of our knowledge, this is the first report about the successful application of a fungal-bacterial consortium in sweet potato cultivation.