Community Structure of Protease-Producing Bacteria Cultivated From Aquaculture Systems: Potential Impact of a Tropical Environment

• Published in: Frontiers in microbiology 2021-02, Vol.12, p.638129-638129
• Main Authors: Wei, Yali, Bu, Jun, Long, Hao, Zhang, Xiang, Cai, Xiaoni, Huang, Aiyou, Ren, Wei, Xie, Zhenyu
• Format: Article
• Language: English
• Subjects: Bacillus hwajinpoensis; enterobacterial repetitive intergenic consensus-polymerase chain reaction; environmental variables; Microbiology; protease-producing bacteria; tropical aquaculture; Vibrio owensii
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2021.638129

Protease-producing bacteria play vital roles in degrading organic matter of aquaculture system, while the knowledge of diversity and bacterial community structure of protease-producing bacteria is limited in this system, especially in the tropical region. Herein, 1,179 cultivable protease-producing bacterial strains that belonged to Actinobacteria, Firmicutes, and Proteobacteria were isolated from tropical aquaculture systems, of which the most abundant genus was , followed by . The diversity and relative abundance of protease-producing bacteria in sediment were generally higher than those in water. Twenty-one genera from sediment and 16 genera from water were identified, of which dominated by in both and dominated by in water were the dominant genera. The unique genera in sediment or water accounted for tiny percentage may play important roles in the stability of community structure. Eighty isolates were clustered into four clusters (ET-1-ET-4) at 58% of similarity by ERIC-PCR (enterobacterial repetitive intergenic consensus-polymerase chain reaction), which was identified as a novel branch of . Additionally, strains belonged to ET-3 and ET-4 were detected in most aquaculture ponds without outbreak of epidemics, indicating that these protease-producing bacteria may be used as potential beneficial bacteria for wastewater purification. Environmental variables played important roles in shaping protease-producing bacterial diversity and community structure in aquaculture systems. In sediment, dissolved oxygen (DO), chemical oxygen demand (COD), and salinity as the main factors positively affected the distributions of dominant genus ( ) and unique genera ( and ), whereas temperature negatively affected that of (except ). In water, as unique genus and were negatively affected by NO -N and NO -N, respectively, whereas pH as the main factor positively affected the distribution of . These findings will lay a foundation for the development of protease-producing bacterial agents for wastewater purification and the construction of an environment-friendly tropical aquaculture model.