Identification of wounding and topping responsive small RNAs in tobacco (Nicotiana tabacum)

MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Over 4,000 miRNAs and several...

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Bibliographic Details
Published in:BMC plant biology 2012-02, Vol.12 (1), p.28-28, Article 28
Main Authors: Tang, She, Wang, Yu, Li, Zefeng, Gui, Yijie, Xiao, Bingguang, Xie, Jiahua, Zhu, Qian-Hao, Fan, Longjiang
Format: Article
Language:English
Subjects:
Flowers & plants
Genetic aspects
Genetic transcription
Genomes
Membranes
MicroRNAs - genetics
Nicotiana - genetics
Nicotine
Physiological aspects
Plant Leaves - genetics
Plant Roots - genetics
RNA
RNA polymerase
RNA, Plant - genetics
RNA, Small Interfering - genetics
Signal transduction
Tobacco
Tobacco (Plant)
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Summary:MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are two major classes of small RNAs. They play important regulatory roles in plants and animals by regulating transcription, stability and/or translation of target genes in a sequence-complementary dependent manner. Over 4,000 miRNAs and several classes of siRNAs have been identified in plants, but in tobacco only computational prediction has been performed and no tobacco-specific miRNA has been experimentally identified. Wounding is believed to induce defensive response in tobacco, but the mechanism responsible for this response is yet to be uncovered. To get insight into the role of small RNAs in damage-induced responses, we sequenced and analysed small RNA populations in roots and leaves from wounding or topping treated tobacco plants. In addition to confirmation of expression of 27 known miRNA families, we identified 59 novel tobacco-specific miRNA members of 38 families and a large number of loci generating phased 21- or 24-nt small RNAs (including ta-siRNAs). A number of miRNAs and phased small RNAs were found to be responsive to wounding or topping treatment. Targets of small RNAs were further surveyed by degradome sequencing. The expression changes of miRNAs and phased small RNAs responsive to wounding or topping and identification of defense related targets for these small RNAs suggest that the inducible defense response in tobacco might be controlled by pathways involving small RNAs.
ISSN:1471-2229
1471-2229
DOI:10.1186/1471-2229-12-28