Simultaneous Quantification of Mixed-Acid Triacylglycerol Positional Isomers and Enantiomers in Palm Oil and Lard by Chiral High-Performance Liquid Chromatography Coupled with Mass Spectrometry

Palm oil and lard are edible fats which are rich in palmitic (P) and oleic acids (O). In this study, triacylglycerol (TAG) positional isomers (symmetric and asymmetric isomers) and enantiomers (asymmetric isomers) in palm oil and lard were quantified simultaneously by using liquid chromatography/mas...

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Bibliographic Details
Published in:Symmetry (Basel) 2020-09, Vol.12 (9), p.1385
Main Authors: Nagai, Toshiharu, Kinoshita, Tetsuaki, Kasamatsu, Erika, Yoshinaga, Kazuaki, Mizobe, Hoyo, Yoshida, Akihiko, Itabashi, Yutaka, Gotoh, Naohiro
Format: Article
Language:English
Subjects:
Asymmetry
chiral HPLC
Chromatography
Enantiomers
Fats
Fatty acids
Gas flow
Glycerol
High performance liquid chromatography
isomer
Isomers
lard
Mass spectrometry
Oils & fats
Palm oil
Physical properties
Scientific imaging
Spectroscopy
triacylglycerol
Triglycerides
Vegetable oils
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Summary:Palm oil and lard are edible fats which are rich in palmitic (P) and oleic acids (O). In this study, triacylglycerol (TAG) positional isomers (symmetric and asymmetric isomers) and enantiomers (asymmetric isomers) in palm oil and lard were quantified simultaneously by using liquid chromatography/mass spectrometry. The CHIRALPAK IF-3 column used in our previous study recognized the difference of TAG isomers consisting of P and O in palm oil and lard, separated sn-OPP/sn-PPO/sn-POP and sn-OPO/sn-OOP/sn-POO into each isomer peak, and enabled the quantification of these TAG isomers with good recovery (95–120%). Although sn-POP and sn-OPO were the major TAGs in palm oil and lard, a comparison of the abundance ratios of TAG enantiomers such as sn-PPO/sn-OPP and sn-OOP/sn-POO revealed that there were slightly more TAG enantiomers with O at the sn-1 position and P at the sn-3 position in palm oil and P at the sn-1 position and O at the sn-3 position in lard. These results were consistent with previous reports for the positional distribution of fatty acids of palm oil and lard. This is the first study that has enabled all TAG isomers consisting of P and O in natural oils and fats to be individually quantified by mass spectrometry.
ISSN:2073-8994
2073-8994
DOI:10.3390/sym12091385