Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines

Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceratio...

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Bibliographic Details
Published in:Makara journal of health research 2016-04, Vol.20 (1), p.11-17
Main Authors: Hamidon, Hanisuhana, Taher, Muhammad, Jaffri, Juliana M, Tg Zakaria, Tg M, Sulaiman, Wan MAW, Susanti, Deny, Ichwan, Solachuddin JA, Zakaria, Zainul A
Format: Article
Language:English
Subjects:
anti-inflammatory
cytotoxicity
garcinia xanthochymus
guttiferae
in vitro
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Summary:Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation.
ISSN:2356-3664
2356-3656
DOI:10.7454/msk.v20i1.5599