Cytotoxic effects of auraptene against a human malignant glioblastoma cell line

Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line. The cellular toxicity was measured by the MTT assay...

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Bibliographic Details
Published in:Avicenna journal of phytomedicine 2019-07, Vol.9 (4), p.334-346
Main Authors: Afshari, Amir R, Karimi Roshan, Mostafa, Soukhtanloo, Mohammad, Ghorbani, Ahmad, Rahmani, Farzad, Jalili-Nik, Mohammad, Vahedi, Mohammad Mahdi, Hoseini, Azar, Sadeghnia, Hamid R, Mollazadeh, Hamid, Mousavi, Seyed Hadi
Format: Article
Language:English
Subjects:
Apoptosis
Auraptene
Brain tumors
Cytotoxicity
Glioblastoma multiforme
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Summary:Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line. The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Ba , , , , , and genes expression, we used real-time polymerase chain reaction (RT-PCR). The results revealed that auraptene reduced the viability of U87 cells concentration- and time-dependently with IC values of 108.9 and 79.17μg/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p
ISSN:2228-7930
2228-7949
DOI:10.22038/ajp.2019.12384