Fluorometric and Colorimetric Method for SARS-CoV-2 Detection Using Designed Aptamer Display Particles

SARS-CoV-2, the virus responsible for the COVID-19 pandemic, has spurred the urgent need for practical diagnostics with high sensitivity and selectivity. Although advanced diagnostic tools have emerged to efficiently control pandemics, they still have costly limitations owing to their reliance on an...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors (Basel) 2024-02, Vol.14 (3), p.113
Main Authors: Park, Ki Sung, Choi, Anna, Park, Tae-In, Pack, Seung Pil
Format: Article
Language:English
Subjects:
Accuracy
Antibodies
Antigens
aptamer display particles
Aptamers
Assaying
Calorimetry
colorimetric aptasensor
Colorimetry
COVID-19
Fabrication
Flow cytometry
Fluorimetry
fluorometric aptasensor
Humans
Infections
Low concentrations
Middle East respiratory syndrome
Pandemics
particle display
Polymerase chain reaction
Proteins
Respiratory diseases
SARS-CoV-2
SARS-CoV-2 aptamer
SARS-CoV-2 spike protein-binding aptamer
Sensitivity
Severe acute respiratory syndrome coronavirus 2
Spike Glycoprotein, Coronavirus
Spike protein
Viral diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SARS-CoV-2, the virus responsible for the COVID-19 pandemic, has spurred the urgent need for practical diagnostics with high sensitivity and selectivity. Although advanced diagnostic tools have emerged to efficiently control pandemics, they still have costly limitations owing to their reliance on antibodies or enzymes and require high-tech equipment. Therefore, there is still a need to develop rapid and low-cost diagnostics with high sensitivity and selectivity. In this study, we generated aptamer display particles (AdP), enabling easy fabrication of a SARS-CoV-2 detection matrix through particle PCR, and applied it to diagnosis using fluorometric and colorimetric assays. We designed two AdPs, C1-AdP and C4-AdP, displayed with SpS1-C1 and SpS1-C4 aptamers, respectively, and showed their high binding ability against SARS-CoV-2 spike protein with a concentration-dependent fluorescence increase. This enabled detection even at low concentrations (0.5 nM). To validate its use as a diagnostic tool for SARS-CoV-2, we designed a sandwich-type assay using two AdPs and high-quality aptamers targeting SARS-CoV-2 pseudoviruses. The fluorometric assay achieved a detection limit of 3.9 × 10 pseudoviruses/mL. The colorimetric assay using an amplification approach exhibited higher sensitivity, with a detection limit of 1 × 10 pseudoviruses/mL, and a broad range of over four orders of magnitude was observed.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios14030113