Establishment of a human embryonic stem cell line, WAe009-A-99, with constitutive expression of the dCas9-p300 fusion protein

CRISPR/Cas9-based transcriptional regulation systems can induce the site-specific activation or repression of endogenous genes. p300 is a transcriptional co-activator that functions as a histone acetyltransferase that regulates gene transcription via chromatin remodeling. Here, we generated a human...

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Bibliographic Details
Published in:Stem cell research 2023-02, Vol.66, p.102986, Article 102986
Main Authors: Kang, Eun-Hye, Kim, Ji-Woo, Noh, Haneul, Park, Seong-Hoon, Park, Han-Jin, Yoon, Seokjoo, Kim, Hyemin
Format: Article
Language:English
Subjects:
Cell Line
CRISPR-Associated Protein 9 - genetics
CRISPR-Cas Systems - genetics
Gene Expression Regulation
Human Embryonic Stem Cells
Humans
Transcription Factors - genetics
Transcriptional Activation
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Summary:CRISPR/Cas9-based transcriptional regulation systems can induce the site-specific activation or repression of endogenous genes. p300 is a transcriptional co-activator that functions as a histone acetyltransferase that regulates gene transcription via chromatin remodeling. Here, we generated a human embryonic stem cell line stably expressing catalytically dead Cas9 (dCas9) fused to the catalytic core domain of human p300 via lentiviral transduction. This cell line can be used for locus-specific histone acetylation in combination with guide RNAs, and is a valuable tool for gene regulation in stem cell research.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.102986