Effects of Extreme Weight Loss on Cardiometabolic Health in Children With Metabolic Syndrome: A Metabolomic Study

• Published in: Frontiers in physiology 2021-09, Vol.12, p.731762-731762
• Main Authors: Liu, Jingxin, Zhu, Lin, Liao, Jing, Liu, Xiaoguang
• Format: Article
• Language: English
• Subjects: cardiometabolic health; children with obesity; exercise; metabolic syndrome; metabolomics; Physiology
• ISSN: 1664-042X; 1664-042X
• DOI: 10.3389/fphys.2021.731762

Objectives: To evaluate the effect of extreme weight loss programs on circulating metabolites and their relationship with cardiometabolic health in children with metabolic syndrome. Methods: This study was a quasi-experimental design with a pretest and post-test. Thirty children with metabolic syndrome and aged 10–17years were recruited to an extreme weight loss program (i.e., exercise combined with diet control). The primary outcomes included plasma metabolites, body composition, and cardiometabolic risk factors. A total of 324 metabolites were quantitatively detected by an ultra-performance liquid chromatography coupled to tandem mass spectrometry system, and the variable importance in the projection (VIP) value of each metabolite was calculated by the orthogonal projection to latent structures discriminant analysis. The fold change (FC) and p value of each metabolite were used to screen differential metabolites with the following values: VIP>1, p value0.25. Pathway enrichment and correlation analyses between metabolites and cardiometabolic risk factors were also performed. Result: A large effect size was observed, presenting a weight loss of −8.9kg (Cohen’s d =1.00, p