Laser emission at 675 nm: In vitro study evidence of a promising role in skin rejuvenation

Light-based therapies have been proven to influence and perhaps reverse skin ageing at clinical, molecular and histologic levels. Laser technology decreases photodamage by promoting collagen type I and III synthesis and enhancing the expression of heat shock protein. Aims: This study aims to assess...

Full description

Saved in:
Bibliographic Details
Published in:Regenerative therapy 2023-03, Vol.22, p.176-180
Main Authors: Magni, Giada, Pieri, Laura, Fusco, Irene, Madeddu, Francesca, Zingoni, Tiziano, Rossi, Francesca
Format: Article
Language:English
Subjects:
675-nm laser wavelength
Confocal microscopy
Laser treatment
Neocollagenesis
Original
Skin aging
Skin resurfacing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Light-based therapies have been proven to influence and perhaps reverse skin ageing at clinical, molecular and histologic levels. Laser technology decreases photodamage by promoting collagen type I and III synthesis and enhancing the expression of heat shock protein. Aims: This study aims to assess different doses of 675 nm irradiation on human dermal fibroblast cells to evaluate the potential therapeutic effects on the rejuvenation process. Methods: This study employed a laser system that emits 675 nm wavelength: 260, 390, 520 and 650 J/cm2 doses were tested on adult human dermal fibroblast cells. Cellular viability, proliferation, and synthesis of type I and III collagen were studied. Results: No dose tested showed effects on cell viability and proliferation at 24 and 48 h from the irradiation. Doses of 260 and 520 J/cm2 causes a significant decrease in type I collagen fluorescence intensity, while 390 J/cm2 elicits a significant increase in type III collagen expression. Conclusions: Our results showed that 675 nm laser irradiation does not affect cell viability while modulating cell proliferation and collagen synthesis in human adult cultured fibroblasts in vitro. These findings suggest that 675 nm laser irradiation potentially plays a role in skin rejuvenation. •Change in type I/III collagen ratio induces premature ageing.•675 nm irradiation can modulate cultured human dermal fibroblasts.•675 nm irradiation directly affects collagen synthesis stimulating neocollagenesis.•Potential role of 675 nm in skin rejuvenation via modulating collagen synthesis.•Light-based therapy can reduce photodamage.
ISSN:2352-3204
2352-3204
DOI:10.1016/j.reth.2023.01.007